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# Clustalw modules

A set of classes to interact with the multiple alignment command
line program clustalw. 

Clustalw is the command line version of the graphical Clustalx 
aligment program.

This requires clustalw available from:


o read
o parse_file
o do_alignment

o ClustalAlignment
o MultipleAlignCL"""

# standard library
import os
import sys

# biopython
from Bio import Alphabet
from Bio.Alphabet import IUPAC
from Bio.Align.Generic import Alignment

def parse_file(file_name, alphabet = IUPAC.unambiguous_dna, debug_level = 0):
    """Parse the given file into a clustal aligment object.
    o file_name - The name of the file to parse.
    o alphabet - The type of alphabet to use for the alignment sequences.
    This should correspond to the type of information contained in the file.
    Defaults to be unambiguous_dna sequence.

    There is a deprecated optional argument debug_level which has no effect.

    # Avoid code duplication by calling Bio.AlignIO to do this for us.
    handle = open(file_name, 'r')
    from Bio import AlignIO
    generic_alignment = AlignIO.read(handle, "clustal")

    #Force this generic alignment into a ClustalAlignment... nasty hack
    if isinstance(alphabet, Alphabet.Gapped) :
        alpha = alphabet
    else :
        alpha = Alphabet.Gapped(alphabet)
    clustal_alignment = ClustalAlignment(alpha)
    clustal_alignment._records = generic_alignment._records
    for record in clustal_alignment._records :
        record.seq.alphabet = alpha
    clustal_alignment._version = generic_alignment._version
    clustal_alignment._star_info = generic_alignment._star_info

    return clustal_alignment

def do_alignment(command_line, alphabet=None):
    """Perform an alignment with the given command line.
    o command_line - A command line object that can give out
    the command line we will input into clustalw.
    o alphabet - the alphabet to use in the created alignment. If not
    specified IUPAC.unambiguous_dna and IUPAC.protein will be used for
    dna and protein alignment respectively.
    o A clustal alignment object corresponding to the created alignment.
    If the alignment type was not a clustal object, None is returned.
    run_clust = os.popen(str(command_line))
    status = run_clust.close()

    # The exit status is the second byte of the termination status
    # TODO - Check this holds on win32...
    value = 0
    if status: value = status / 256
    # check the return value for errors, as on 1.81 the return value
    # from Clustalw is actually helpful for figuring out errors
    # 1 => bad command line option
    if value == 1:
        raise ValueError("Bad command line option in the command: %s"
                         % str(command_line))
    # 2 => can't open sequence file
    elif value == 2:
        raise IOError("Cannot open sequence file %s"
                      % command_line.sequence_file)
    # 3 => wrong format in sequence file
    elif value == 3:
        raise IOError("Sequence file %s has an invalid format."
                      % command_line.sequence_file)
    # 4 => sequence file only has one sequence
    elif value == 4:
        raise IOError("Sequence file %s has only one sequence present."
                      % command_line.sequence_file)

    # if an output file was specified, we need to grab it
    if command_line.output_file:
        out_file = command_line.output_file
        out_file = os.path.splitext(command_line.sequence_file)[0] + '.aln'

    # if we can't deal with the format, just return None
    if command_line.output_type and command_line.output_type != 'CLUSTAL':
        return None
    # otherwise parse it into a ClustalAlignment object
        if not alphabet:
            alphabet = (IUPAC.unambiguous_dna, IUPAC.protein)[
                command_line.type == 'PROTEIN']
        # check if the outfile exists before parsing
        if not(os.path.exists(out_file)):
            raise IOError("Output .aln file %s not produced, commandline: %s"
                          % (out_file, command_line))
        return parse_file(out_file, alphabet)

00128 class ClustalAlignment(Alignment):
    """Work with the clustal aligment format.

    This format is the default output from clustal -- these files normally
    have an extension of .aln.
    # the default version to use if one isn't set
    DEFAULT_VERSION = '1.81'
00137     def __init__(self, alphabet = Alphabet.Gapped(IUPAC.ambiguous_dna)):
        Alignment.__init__(self, alphabet)

        # represent all of those stars in the aln output format
        self._star_info = ''
        self._version = ''

00145     def __str__(self):
        """Print out the alignment so it looks pretty.

        The output produced from this should also be formatted in valid
        clustal format.
        # Avoid code duplication by calling Bio.AlignIO to do this for us.
        from Bio import AlignIO
        from StringIO import StringIO
        handle = StringIO()
        AlignIO.write([self], handle, "clustal")
        return handle.read()

00160     def _add_star_info(self, stars):
        """Add all of the stars, which indicate consensus sequence.
        self._star_info = stars

00165     def _add_version(self, version):
        """Add the version information about the clustal file being read.
        self._version = version

00171 class MultipleAlignCL:
    """Represent a clustalw multiple alignment command line.

    This is meant to make it easy to code the command line options you
    want to submit to clustalw.

    Clustalw has a ton of options and things to do but this is set up to
    represent a clustalw mutliple alignment.

    Warning: I don't use all of these options personally, so if you find
    one to be broken for any reason, please let us know!
    # set the valid options for different parameters

00192     def __init__(self, sequence_file, command = 'clustalw'):
        """Initialize some general parameters that can be set as attributes.

        o sequence_file - The file to read the sequences for alignment from.
        o command - The command used to run clustalw. This defaults to
        just 'clustalw' (ie. assumes you have it on your path somewhere).
        General attributes that can be set:
        o is_quick - if set as 1, will use a fast algorithm to create
        the alignment guide tree.
        o allow_negative - allow negative values in the alignment matrix.

        Multiple alignment attributes that can be set as attributes:
        o gap_open_pen - Gap opening penalty
        o gap_ext_pen - Gap extension penalty
        o is_no_end_pen - A flag as to whether or not there should be a gap
        separation penalty for the ends.
        o gap_sep_range - The gap separation penalty range.
        o is_no_pgap - A flag to turn off residue specific gaps
        o is_no_hgap - A flag to turn off hydrophilic gaps
        o h_gap_residues - A list of residues to count a hydrophilic
        o max_div - A percent identity to use for delay (? - I don't undertand
        o trans_weight - The weight to use for transitions
        self.sequence_file = sequence_file
        self.command = command
        self.is_quick = None
        self.allow_negative = None

        self.gap_open_pen = None
        self.gap_ext_pen = None
        self.is_no_end_pen = None
        self.gap_sep_range = None
        self.is_no_pgap = None
        self.is_no_hgap = None
        self.h_gap_residues = []
        self.max_div = None
        self.trans_weight = None

        # other attributes that should be set via various functions
        # 1. output parameters
        self.output_file = None
        self.output_type = None
        self.output_order = None
        self.change_case = None
        self.add_seqnos = None

        # 2. a guide tree to use
        self.guide_tree = None
        self.new_tree = None

        # 3. matrices
        self.protein_matrix = None
        self.dna_matrix = None

        # 4. type of residues
        self.type = None

00253     def __str__(self):
        """Write out the command line as a string."""

        if sys.platform <> "win32" :
            #On Linux with clustalw 1.83, you can do:
            #clustalw input.faa
            #clustalw /full/path/input.faa
            #clustalw -INFILE=input.faa
            #clustalw -INFILE=/full/path/input.faa
            #Note these fail (using DOS style slashes):
            #clustalw /INFILE=input.faa
            #clustalw /INFILE=/full/path/input.faa
            #To keep things simple, and follow the original
            #behaviour of Bio.Clustalw use this:
            cline = self.command + " " + self.sequence_file
        else :
            #On Windows XP with clustalw.exe 1.83, these work at
            #the command prompt:
            #clustalw.exe input.faa
            #clustalw.exe /INFILE=input.faa
            #clustalw.exe /INFILE="input.faa"
            #clustalw.exe /INFILE="with space.faa"
            #clustalw.exe /INFILE=C:\full\path\input.faa
            #clustalw.exe /INFILE="C:\full path\with spaces.faa"
            #Sadly these fail:
            #clustalw.exe "input.faa"
            #clustalw.exe "with space.faa"
            #clustalw.exe C:\full\path\input.faa
            #clustalw.exe "C:\full path\with spaces.faa"
            #These also fail but a minus/dash does seem to
            #work with other options (!):
            #clustalw.exe -INFILE=input.faa
            #clustalw.exe -INFILE=C:\full\path\input.faa
            #Also these fail:
            #clustalw.exe "/INFILE=input.faa"
            #clustalw.exe "/INFILE=C:\full\path\input.faa"
            #Thanks to Emanuel Hey for flagging this on the mailing list.
            #In addtion, both self.command and self.sequence_file
            #may contain spaces, so should be quoted. But clustalw
            #is fussy.
            if self.command.count(" ") > 0 :
                cline = '"%s"' % self.command
            else :
                cline = self.command
            if self.sequence_file.count(" ") > 0 :
                cline += ' /INFILE="%s"' % self.sequence_file
            else :
                cline += ' /INFILE=%s' % self.sequence_file

        # general options
        if self.type:
            cline += " -TYPE=%s" % self.type
        if self.is_quick == 1:
            #Some versions of clustalw are case sensitive,
            #and require -quicktree rather than -QUICKTREE
            cline += " -quicktree"
        if self.allow_negative == 1:
            cline += " -NEGATIVE"

        # output options
        if self.output_file:
            cline += " -OUTFILE=%s" % self.output_file
        if self.output_type:
            cline += " -OUTPUT=%s" % self.output_type
        if self.output_order:
            cline += " -OUTORDER=%s" % self.output_order
        if self.change_case:
            cline += " -CASE=%s" % self.change_case
        if self.add_seqnos:
            cline += " -SEQNOS=%s" % self.add_seqnos
        if self.new_tree:
            # clustal does not work if -align is written -ALIGN
            cline += " -NEWTREE=%s -align" % self.new_tree

        # multiple alignment options
        if self.guide_tree:
            cline += " -USETREE=%s" % self.guide_tree
        if self.protein_matrix:
            cline += " -MATRIX=%s" % self.protein_matrix
        if self.dna_matrix:
            cline += " -DNAMATRIX=%s" % self.dna_matrix
        if self.gap_open_pen:
            cline += " -GAPOPEN=%s" % self.gap_open_pen
        if self.gap_ext_pen:
            cline += " -GAPEXT=%s" % self.gap_ext_pen
        if self.is_no_end_pen == 1:
            cline += " -ENDGAPS"
        if self.gap_sep_range:
            cline += " -GAPDIST=%s" % self.gap_sep_range
        if self.is_no_pgap == 1:
            cline += " -NOPGAP"
        if self.is_no_hgap == 1:
            cline += " -NOHGAP"
        if len(self.h_gap_residues) != 0:
            # stick the list of residues together as one big list o' residues
            residue_list = ''
            for residue in self.h_gap_residues:
                residue_list = residue_list + residue
            cline += " -HGAPRESIDUES=%s" % residue_list
        if self.max_div:
            cline += " -MAXDIV=%s" % self.max_div
        if self.trans_weight:
            cline += " -TRANSWEIGHT=%s" % self.trans_weight

        return cline

00368     def set_output(self, output_file, output_type = None, output_order = None,
                   change_case = None, add_seqnos = None):
        """Set the output parameters for the command line.
        self.output_file = output_file

        if output_type:
            output_type = output_type.upper()
            if output_type not in self.OUTPUT_TYPES:
                raise ValueError("Invalid output type %s. Valid choices are %s"
                                 % (output_type, self.OUTPUT_TYPES))
                self.output_type = output_type

        if output_order:
            output_order = output_order.upper()
            if output_order not in self.OUTPUT_ORDER:
                raise ValueError("Invalid output order %s. Valid choices are %s"
                                 % (output_order, self.OUTPUT_ORDER))
                self.output_order = output_order

        if change_case:
            change_case = change_case.upper()
            if output_type != "GDE":
                raise ValueError("Change case only valid for GDE output.")
            elif change_case not in self.CHANGE_CASE:
                raise ValueError("Invalid change case %s. Valid choices are %s"
                                 % (change_case, self.CHANGE_CASE))
                self.change_case = change_case

        if add_seqnos:
            add_seqnos = add_seqnos.upper()
            if output_type:
                raise ValueError("Add SeqNos only valid for CLUSTAL output.")
            elif add_seqnos not in self.OUTPUT_SEQNOS:
                raise ValueError("Invalid seqnos option %s. Valid choices: %s"
                                 % (add_seqnos, self.OUTPUT_SEQNOS))
                self.add_seqnos = add_seqnos

00410     def set_guide_tree(self, tree_file):
        """Provide a file to use as the guide tree for alignment.

        o IOError - If the tree_file doesn't exist."""
        if not(os.path.exists(tree_file)):
            raise IOError("Could not find the guide tree file %s." %
            self.guide_tree = tree_file

00421     def set_new_guide_tree(self, tree_file):
        """Set the name of the guide tree file generated in the alignment.
        self.new_tree = tree_file
00426     def set_protein_matrix(self, protein_matrix):
        """Set the type of protein matrix to use.

        Protein matrix can be either one of the defined types (blosum, pam,
        gonnet or id) or a file with your own defined matrix.
        if protein_matrix.upper() in self.PROTEIN_MATRIX:
            self.protein_matrix = protein_matrix.upper()
        elif os.path.exists(protein_matrix):
            self.protein_matrix = protein_matrix
            raise ValueError("Invalid matrix %s. Options are %s or a file." %
                             (protein_matrix.upper(), self.PROTEIN_MATRIX))

00440     def set_dna_matrix(self, dna_matrix):
        """Set the type of DNA matrix to use.

        The dna_matrix can either be one of the defined types (iub or clustalw)
        or a file with the matrix to use."""
        if dna_matrix.upper() in self.DNA_MATRIX:
            self.dna_matrix = dna_matrix.upper()
        elif os.path.exists(dna_matrix):
            self.dna_matrix = dna_matrix
            raise ValueError("Invalid matrix %s. Options are %s or a file." %
                             (dna_matrix, self.DNA_MATRIX))

00453     def set_type(self, residue_type):
        """Set the type of residues within the file.

        Clustal tries to guess whether the info is protein or DNA based on
        the number of GATCs, but this can be wrong if you have a messed up
        protein or DNA you are working with, so this allows you to set it
        residue_type = residue_type.upper()
        if residue_type in self.RESIDUE_TYPES:
            self.type = residue_type
            raise ValueError("Invalid residue type %s. Valid choices are %s"
                             % (residue_type, self.RESIDUE_TYPES))

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