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00001 """
Contains classes to deal with generic sequence alignment stuff not
specific to a particular program or format.

o Alignment

# standard library
import string

# biopython
from Bio.Seq import Seq
from Bio.SeqRecord import SeqRecord
from Bio import Alphabet
from Bio.Alphabet import IUPAC

00018 class Alignment:
    """Represent a set of alignments.

    This is a base class to represent alignments, which can be subclassed
    to deal with an alignment in a specific format.
00024     def __init__(self, alphabet):
        """Initialize a new Alignment object.

        o alphabet - The alphabet to use for the sequence objects that are
        created. This alphabet must be a gapped type.
        self._alphabet = alphabet
        # hold everything at a list of seq record objects
        self._records = []

00035     def _str_line(self, record) :
        """Returns a truncated string representation of a SeqRecord.

        This is a PRIVATE function used by the __str__ method.
        if len(record.seq) <= 50 :
            return "%s %s" % (record.seq, record.id)
        else :
            return "%s...%s %s" \
                   % (record.seq[:44], record.seq[-3:], record.id)

00046     def __str__(self) :
        """Returns a multi-line string summary of the alignment.

        This output is intended to be readable, but large alignments are
        shown truncated.  A maximum of 20 rows (sequences) and 50 columns
        are shown, with the record identifiers.  This should fit nicely on a
        single screen.  e.g.

        DNAAlphabet() alignment with 3 rows and 14 columns
        ACGATGAGCTAGCT Gamma        
        rows = len(self._records)
        lines = ["%s alignment with %i rows and %i columns" \
                 % (str(self._alphabet), rows, self.get_alignment_length())]
        if rows <= 20 :
            lines.extend([self._str_line(rec) for rec in self._records])
        else :
            lines.extend([self._str_line(rec) for rec in self._records[:18]])
        return "\n".join(lines)

00070     def __repr__(self) :
        """Returns a representation of the object for debugging.

        The representation cannot be used with eval() to recreate the object,
        which is usually possible with simple python ojects.  For example:

        <Bio.Align.Generic.Alignment instance (2 records of length 14,
        SingleLetterAlphabet()) at a3c184c>

        The hex string is the memory address of the object, see help(id).
        This provides a simple way to visually distinguish alignments of
        the same size.
        return "<%s instance (%i records of length %i, %s) at %x>" % \
               (self.__class__, len(self._records),
                self.get_alignment_length(), repr(self._alphabet), id(self))
        #This version is useful for doing eval(repr(alignment)),
        #but it can be VERY long:
        #return "%s(%s, %s)" \
        #       % (self.__class__, repr(self._records), repr(self._alphabet))

00091     def get_all_seqs(self):
        """Return all of the sequences involved in the alignment.

        The return value is a list of SeqRecord objects.
        return self._records

00098     def __iter__(self) :
        """Iterate over alignment rows as SeqRecord objects


        for record in align :
            print record.id
            print record.seq
        return iter(self._records) 

00109     def get_seq_by_num(self, number):
        """Retrieve a sequence by row number.

        o A Seq object for the requested sequence.

        o IndexError - If the specified number is out of range.
        return self._records[number].seq

00120     def get_alignment_length(self):
        """Return the maximum length of the alignment.

        All objects in the alignment should (hopefully) have the same
        length. This function will go through and find this length
        by finding the maximum length of sequences in the alignment.
        max_length = 0

        for record in self._records:
            if len(record.seq) > max_length:
                max_length = len(record.seq)

        return max_length

00135     def add_sequence(self, descriptor, sequence, start = None, end = None,
                     weight = 1.0):
        """Add a sequence to the alignment.

        This doesn't do any kind of alignment, it just adds in the sequence
        object, which is assumed to be prealigned with the existing

        o descriptor - The descriptive id of the sequence being added.
                       This will be used as the resulting SeqRecord's
                       .id property (and, for historical compatibility,
                       also the .description property)
        o sequence - A string with sequence info.
        o start - You can explicitly set the start point of the sequence.
        This is useful (at least) for BLAST alignments, which can just
        be partial alignments of sequences.
        o end - Specify the end of the sequence, which is important
        for the same reason as the start.
        o weight - The weight to place on the sequence in the alignment.
        By default, all sequences have the same weight. (0.0 => no weight,
        1.0 => highest weight)
        new_seq = Seq(sequence, self._alphabet)

        #We are now effectively using the SeqRecord's .id as
        #the primary identifier (e.g. in Bio.SeqIO) so we should
        #populate it with the descriptor.
        #For backwards compatibility, also store this in the
        #SeqRecord's description property.
        new_record = SeqRecord(new_seq,
                               id = descriptor,
                               description = descriptor)

        # hack! We really need to work out how to deal with annotations
        # and features in biopython. Right now, I'll just use the
        # generic annotations dictionary we've got to store the start
        # and end, but we should think up something better. I don't know
        # if I'm really a big fan of the LocatableSeq thing they've got
        # in BioPerl, but I'm not positive what the best thing to do on
        # this is...
        if start:
            new_record.annotations['start'] = start
        if end:
            new_record.annotations['end'] = end

        # another hack to add weight information to the sequence
        new_record.annotations['weight'] = weight

00186     def get_column(self,col):
        """Returns a string containing a given column."""
        col_str = ''
        assert col >= 0 and col <= self.get_alignment_length()
        for rec in self._records:
            col_str += rec.seq[col]
        return col_str
if __name__ == "__main__" :
    print "Mini self test..."

    a = Alignment(Alphabet.generic_dna)
    a.add_sequence("Alpha", raw_data[0], weight=2)
    a.add_sequence("Beta",  raw_data[1])
    a.add_sequence("Gamma", raw_data[2])

    print "str(a):"
    print str(a)
    print "repr(a):"
    print repr(a)

    #Iterating over the rows...
    for rec in a :
        assert isinstance(rec, SeqRecord)
    for r,rec in enumerate(a) :
        assert isinstance(rec, SeqRecord)
        assert raw_data[r] == rec.seq.tostring()
        if r==0 : assert rec.annotations['weight']==2
    print "Alignment iteraction as SeqRecord OK"

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