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test_SeqIO.py
# Copyright 2007-2010 by Peter Cock.  All rights reserved.
# This code is part of the Biopython distribution and governed by its
# license.  Please see the LICENSE file that should have been included
# as part of this package.

import os

from Bio import SeqIO
from Bio import AlignIO
from Bio.SeqRecord import SeqRecord
from Bio.Seq import Seq, UnknownSeq
from StringIO import StringIO
from Bio import Alphabet
from Bio.Align import MultipleSeqAlignment

try:
    #This is in Python 2.6+, but we need it on Python 3
    from io import BytesIO
except ImportError:
    BytesIO = StringIO

import warnings
def send_warnings_to_stdout(message, category, filename, lineno,
                                file=None, line=None):
    #TODO - Have Biopython DataLossWarning?
    if category in [UserWarning]:
        print "%s - %s" % (category.__name__, message)
warnings.showwarning = send_warnings_to_stdout


protein_alphas = [Alphabet.generic_protein]
dna_alphas = [Alphabet.generic_dna]
rna_alphas = [Alphabet.generic_rna]
nucleotide_alphas = [Alphabet.generic_nucleotide,
                     Alphabet.Gapped(Alphabet.generic_nucleotide)]
no_alpha_formats = ["fasta","clustal","phylip","phylip-relaxed","tab","ig",
                    "stockholm","emboss", "fastq","fastq-solexa",
                    "fastq-illumina","qual"]
possible_unknown_seq_formats = ["qual", "genbank", "gb", "embl", "imgt"]

#List of formats including alignment only file formats we can read AND write.
#The list is initially hard coded to preserve the original order of the unit
#test output, with any new formats added since appended to the end.
test_write_read_alignment_formats = ["fasta","clustal","phylip","stockholm",
                                     "phylip-relaxed"]
for format in sorted(SeqIO._FormatToWriter):
    if format not in test_write_read_alignment_formats:
        test_write_read_alignment_formats.append(format)
for format in sorted(AlignIO._FormatToWriter):
    if format not in test_write_read_alignment_formats:
        test_write_read_alignment_formats.append(format)
test_write_read_alignment_formats.remove("gb") #an alias for genbank
test_write_read_alignment_formats.remove("fastq-sanger") #an alias for fastq

# test_files is a list of tuples containing:
# - string:  file format
# - boolean: alignment (requires all seqs be same length)
# - string:  relative filename
# - integer: number of sequences

test_files = [ \
    ("sff",    False, 'Roche/E3MFGYR02_random_10_reads.sff', 10),
#Following examples are also used in test_Clustalw.py
    ("clustal",True,  'Clustalw/cw02.aln', 2),
    ("clustal",True,  'Clustalw/opuntia.aln', 7),
    ("clustal",True,  'Clustalw/hedgehog.aln', 5),
    ("clustal",True,  'Clustalw/odd_consensus.aln', 2),
#Following nucleic examples are also used in test_SeqIO_FastaIO.py
    ("fasta",  False, 'Fasta/lupine.nu', 1),
    ("fasta",  False, 'Fasta/elderberry.nu', 1),
    ("fasta",  False, 'Fasta/phlox.nu', 1),
    ("fasta",  False, 'Fasta/centaurea.nu', 1),
    ("fasta",  False, 'Fasta/wisteria.nu', 1),
    ("fasta",  False, 'Fasta/sweetpea.nu', 1),
    ("fasta",  False, 'Fasta/lavender.nu', 1),
#Following protein examples are also used in test_SeqIO_FastaIO.py
    ("fasta",  False, 'Fasta/aster.pro', 1),
    ("fasta",  False, 'Fasta/loveliesbleeding.pro', 1),
    ("fasta",  False, 'Fasta/rose.pro', 1),
    ("fasta",  False, 'Fasta/rosemary.pro', 1),
#Following examples are also used in test_BioSQL_SeqIO.py
    ("fasta",  False, 'Fasta/f001', 1), #Protein
    ("fasta",  False, 'Fasta/f002', 3), #DNA
    #("fasta", False, 'Fasta/f003', 2), #Protein with comments
    ("fasta",  False, 'Fasta/fa01', 2), #Protein with gaps
#Following are also used in test_SeqIO_features.py, see also NC_005816.gb
    ("fasta",  False, 'GenBank/NC_005816.fna', 1),
    ("fasta",  False, 'GenBank/NC_005816.ffn', 10),
    ("fasta",  False, 'GenBank/NC_005816.faa', 10),
    ("fasta",  False, 'GenBank/NC_000932.faa', 85),
    ("tab",  False, 'GenBank/NC_005816.tsv', 10), # FASTA -> Tabbed
#Following examples are also used in test_GFF.py
    ("fasta",  False, 'GFF/NC_001802.fna', 1), #upper case
    ("fasta",  False, 'GFF/NC_001802lc.fna', 1), #lower case
    ("fasta",  True,  'GFF/multi.fna', 3), #Trivial nucleotide alignment
#Following example is also used in test_registry.py
    ("fasta",  False, 'Registry/seqs.fasta', 2), #contains blank line
#Following example is also used in test_Nexus.py
    ("nexus",  True,  'Nexus/test_Nexus_input.nex', 9),
#Following examples are also used in test_SwissProt.py
    ("swiss",  False, 'SwissProt/sp001', 1),
    ("swiss",  False, 'SwissProt/sp002', 1),
    ("swiss",  False, 'SwissProt/sp003', 1),
    ("swiss",  False, 'SwissProt/sp004', 1),
    ("swiss",  False, 'SwissProt/sp005', 1),
    ("swiss",  False, 'SwissProt/sp006', 1),
    ("swiss",  False, 'SwissProt/sp007', 1),
    ("swiss",  False, 'SwissProt/sp008', 1),
    ("swiss",  False, 'SwissProt/sp009', 1),
    ("swiss",  False, 'SwissProt/sp010', 1),
    ("swiss",  False, 'SwissProt/sp011', 1),
    ("swiss",  False, 'SwissProt/sp012', 1),
    ("swiss",  False, 'SwissProt/sp013', 1),
    ("swiss",  False, 'SwissProt/sp014', 1),
    ("swiss",  False, 'SwissProt/sp015', 1),
    ("swiss",  False, 'SwissProt/sp016', 1),
#Following example is also used in test_registry.py
    ("swiss",  False, 'Registry/EDD_RAT.dat', 1),
#Following examples are also used in test_Uniprot.py
    ("uniprot-xml",  False, 'SwissProt/uni001', 1),
    ("uniprot-xml",  False, 'SwissProt/uni002', 3),
    ("uniprot-xml",  False, 'SwissProt/Q13639.xml', 1),
    ("swiss",    False, 'SwissProt/Q13639.txt', 1),
#Following examples are also used in test_GenBank.py
    ("genbank",False, 'GenBank/noref.gb', 1),
    ("genbank",False, 'GenBank/cor6_6.gb', 6),
    ("genbank",False, 'GenBank/iro.gb', 1),
    ("genbank",False, 'GenBank/pri1.gb', 1),
    ("genbank",False, 'GenBank/arab1.gb', 1),
    ("genbank",False, 'GenBank/protein_refseq.gb', 1), #Old version
    ("genbank",False, 'GenBank/protein_refseq2.gb', 1), #Revised version
    ("genbank",False, 'GenBank/extra_keywords.gb', 1),
    ("genbank",False, 'GenBank/one_of.gb', 1),
    ("genbank",False, 'GenBank/NT_019265.gb', 1), #contig, no sequence
    ("genbank",False, 'GenBank/origin_line.gb', 1),
    ("genbank",False, 'GenBank/blank_seq.gb', 1),
    ("genbank",False, 'GenBank/dbsource_wrap.gb', 1),
    ("genbank",False, 'GenBank/NC_005816.gb', 1), #See also AE017046.embl
    ("genbank",False, 'GenBank/NC_000932.gb', 1),
    ("genbank",False, 'GenBank/pBAD30.gb', 1), #Odd LOCUS line from Vector NTI
# The next example is a truncated copy of gbvrl1.seq from
# ftp://ftp.ncbi.nih.gov/genbank/gbvrl1.seq.gz
# This includes an NCBI header, and the first three records:
    ("genbank",False, 'GenBank/gbvrl1_start.seq', 3),
#Following files are also used in test_GFF.py
    ("genbank",False, 'GFF/NC_001422.gbk', 1),
#Following files are currently only used here or in test_SeqIO_index.py:
    ("embl",   False, 'EMBL/epo_prt_selection.embl', 9), #proteins
    ("embl",   False, 'EMBL/TRBG361.embl', 1),
    ("embl",   False, 'EMBL/DD231055_edited.embl', 1),
    ("embl",   False, 'EMBL/SC10H5.embl', 1), # Pre 2006 style ID line
    ("embl",   False, 'EMBL/U87107.embl', 1), # Old ID line with SV line
    ("embl",   False, 'EMBL/AAA03323.embl', 1), # 2008, PA line but no AC
    ("embl",   False, 'EMBL/AE017046.embl', 1), #See also NC_005816.gb
    ("embl",   False, 'EMBL/Human_contigs.embl', 2), #contigs, no sequences
    ("embl",   False, 'EMBL/A04195.imgt', 1), # features over indented for EMBL
    ("imgt",   False, 'EMBL/A04195.imgt', 1), # features over indented for EMBL
    ("stockholm", True,  'Stockholm/simple.sth', 2),
    ("stockholm", True,  'Stockholm/funny.sth', 5),
#Following PHYLIP files are currently only used here and in test_AlignIO.py,
#and are mostly from Joseph Felsenstein's PHYLIP v3.6 documentation:
    ("phylip", True,  'Phylip/reference_dna.phy', 6),
    ("phylip", True,  'Phylip/reference_dna2.phy', 6),
    ("phylip", True,  'Phylip/hennigian.phy', 10),
    ("phylip", True,  'Phylip/horses.phy', 10),
    ("phylip", True,  'Phylip/random.phy', 10),
    ("phylip", True,  'Phylip/interlaced.phy', 3),
    ("phylip", True,  'Phylip/interlaced2.phy', 4),
#Following are EMBOSS simple or pairs format alignments
    ("emboss", True,  'Emboss/alignret.txt', 4),
    ("emboss", False, 'Emboss/needle.txt', 10),
    ("emboss", True,  'Emboss/water.txt', 2),
#Following PHD (PHRAP) sequencing files are also used in test_Phd.py
    ("phd", False, 'Phd/phd1', 3),
    ("phd", False, 'Phd/phd2', 1),
    ("phd", False, 'Phd/phd_solexa', 2),
    ("phd", False, 'Phd/phd_454', 1),
#Following ACE assembly files are also used in test_Ace.py
    ("ace", False, 'Ace/contig1.ace', 2),
    ("ace", False, 'Ace/consed_sample.ace', 1),
    ("ace", False, 'Ace/seq.cap.ace', 1),
#Following IntelliGenetics / MASE files are also used in test_intelligenetics.py
    ("ig",  False, 'IntelliGenetics/TAT_mase_nuc.txt', 17),
    ("ig",  True,  'IntelliGenetics/VIF_mase-pro.txt', 16),
    #This next file is a MASE alignment but sequence O_ANT70 is shorter than
    #the others (so as an alignment will fail).  Perhaps MASE doesn't
    #write trailing gaps?
    ("ig",  False,  'IntelliGenetics/vpu_nucaligned.txt', 9),
#Following NBRD-PIR files are used in test_nbrf.py
    ("pir", False, 'NBRF/B_nuc.pir', 444),
    ("pir", False, 'NBRF/Cw_prot.pir', 111),
    ("pir", False, 'NBRF/DMA_nuc.pir', 4),
    ("pir", False, 'NBRF/DMB_prot.pir', 6),
    ("pir", True,  'NBRF/clustalw.pir', 2),
#Following quality files are also used in the Bio.SeqIO.QualityIO doctests:
    ("fasta", True, 'Quality/example.fasta', 3),
    ("qual",  False,'Quality/example.qual',  3),
    ("fastq", True, 'Quality/example.fastq', 3),
    ("fastq", True, 'Quality/tricky.fastq', 4),
    ("fastq", False,'Quality/sanger_faked.fastq', 1),
    ("fastq", False,'Quality/sanger_93.fastq', 1),
    ("fastq-illumina", False,'Quality/illumina_faked.fastq', 1),
    ("fastq-solexa", False, 'Quality/solexa_faked.fastq', 1),
    ("fastq-solexa", True, 'Quality/solexa_example.fastq', 5),
#Following examples are also used in test_SeqXML.py
    ("seqxml", False, 'SeqXML/dna_example.xml', 4),
    ("seqxml", False, 'SeqXML/rna_example.xml', 5),
    ("seqxml", False, 'SeqXML/protein_example.xml', 5),
#Following examples are also used in test_SeqIO_AbiIO.py
    ("abi", False, 'Abi/310.ab1', 1),
    ("abi", False, 'Abi/3100.ab1', 1),
    ("abi", False, 'Abi/3730.ab1', 1),
    ]

00215 class ForwardOnlyHandle(object):
    """Mimic a network handle without seek and tell methods etc."""
    def __init__(self, handle):
        self._handle = handle

    def __iter__(self):
        return iter(self._handle)

    def read(self, length=None):
        if length is None:
            return self._handle.read()
        else:
            return self._handle.read(length)

    def readline(self):
        return self._handle.readline()

    def close(self):
        return self._handle.close()

def compare_record(record_one, record_two):
    """This is meant to be a strict comparison for exact agreement..."""
    assert isinstance(record_one, SeqRecord)
    assert isinstance(record_two, SeqRecord)
    assert record_one.seq is not None
    assert record_two.seq is not None
    if record_one.id != record_two.id:
        return False
    if record_one.name != record_two.name:
        return False
    if record_one.description != record_two.description:
        return False
    if len(record_one) != len(record_two):
        return False
    if isinstance(record_one.seq, UnknownSeq) \
    and isinstance(record_two.seq, UnknownSeq):
        #Jython didn't like us comparing the string of very long UnknownSeq
        #object (out of heap memory error)
        if record_one.seq._character != record_two.seq._character:
            return False
    elif record_one.seq.tostring() != record_two.seq.tostring():
        return False
    #TODO - check features and annotation (see code for BioSQL tests)
    for key in set(record_one.letter_annotations).intersection( \
                   record_two.letter_annotations):
        if record_one.letter_annotations[key] != \
           record_two.letter_annotations[key]:
            return False
    return True

def record_summary(record, indent=" "):
    """Returns a concise summary of a SeqRecord object as a string"""
    if record.id == record.name:
        answer = "%sID and Name='%s',\n%sSeq='" % (indent, record.id, indent)
    else:
        answer = "%sID = '%s', Name='%s',\n%sSeq='" % (indent, record.id, record.name, indent)
    if record.seq is None:
        answer += "None"
    else:
        if len(record.seq) > 50:
            answer += record.seq[:40].tostring() + "..." + record.seq[-7:].tostring()
        else:
            answer += record.seq.tostring()
        answer += "', length=%i" % (len(record.seq))
    return answer

def col_summary(col_text):
    if len(col_text) < 65:
        return col_text
    else:
        return col_text[:60] + "..." + col_text[-5:]

def alignment_summary(alignment, index=" "):
    """Returns a concise summary of an Alignment object as a string"""
    answer = []
    alignment_len = alignment.get_alignment_length()
    rec_count = len(alignment)
    for i in range(min(5,alignment_len)):
        answer.append(index + col_summary(alignment.get_column(i)) \
                            + " alignment column %i" % i)
    if alignment_len > 5:
        i = alignment_len - 1
        answer.append(index + col_summary("|" * rec_count) \
                            + " ...")
        answer.append(index + col_summary(alignment.get_column(i)) \
                            + " alignment column %i" % i)
    return "\n".join(answer)


def check_simple_write_read(records, indent=" "):
    #print indent+"Checking we can write and then read back these records"
    for format in test_write_read_alignment_formats:
        if format not in possible_unknown_seq_formats \
        and isinstance(records[0].seq, UnknownSeq) \
        and len(records[0].seq) > 100:
           #Skipping for speed.  Some of the unknown sequences are
           #rather long, and it seems a bit pointless to record them.
           continue
        print indent+"Checking can write/read as '%s' format" % format

        #Going to write to a handle...
        if format in SeqIO._BinaryFormats:
            handle = BytesIO()
        else:
            handle = StringIO()

        try:
            c = SeqIO.write(sequences=records, handle=handle, format=format)
            assert c == len(records)
        except (TypeError, ValueError), e:
            #This is often expected to happen, for example when we try and
            #write sequences of different lengths to an alignment file.
            if "len()" in str(e):
                #Python 2.4.3,
                #>>> len(None)
                #...
                #TypeError: len() of unsized object
                #
                #Python 2.5.2,
                #>>> len(None)
                #...
                #TypeError: object of type 'NoneType' has no len()
                print "Failed: Probably len() of None"
            else:
                print indent+"Failed: %s" % str(e)
            assert format != t_format, \
                   "Should be able to re-write in the original format!"
            #Carry on to the next format:
            continue

        handle.flush()
        handle.seek(0)
        #Now ready to read back from the handle...
        try:
            records2 = list(SeqIO.parse(handle=handle, format=format))
        except ValueError, e:
            #This is BAD.  We can't read our own output.
            #I want to see the output when called from the test harness,
            #run_tests.py (which can be funny about new lines on Windows)
            handle.seek(0)
            raise ValueError("%s\n\n%s\n\n%s" \
                              % (str(e), repr(handle.read()), repr(records)))

        assert len(records2) == t_count
        for r1, r2 in zip(records, records2):
            #Check the bare minimum (ID and sequence) as
            #many formats can't store more than that.
            assert len(r1) == len(r2)

            #Check the sequence
            if format in ["gb", "genbank", "embl", "imgt"]:
                #The GenBank/EMBL parsers will convert to upper case.
                if isinstance(r1.seq, UnknownSeq) \
                and isinstance(r2.seq, UnknownSeq):
                    #Jython didn't like us comparing the string of very long
                    #UnknownSeq object (out of heap memory error)
                    assert r1.seq._character.upper() == r2.seq._character
                else:
                    assert r1.seq.tostring().upper() == r2.seq.tostring()
            elif format == "qual":
                assert isinstance(r2.seq, UnknownSeq)
                assert len(r2) == len(r1)
            else:
                assert r1.seq.tostring() == r2.seq.tostring()
            #Beware of different quirks and limitations in the
            #valid character sets and the identifier lengths!
            if format=="phylip":
                assert r1.id.replace("[","").replace("]","")[:10] == r2.id, \
                       "'%s' vs '%s'" % (r1.id, r2.id)
            elif format=="phylip-relaxed":
                assert r1.id.replace(" ", "").replace(':', '|') == r2.id, \
                        "'%s' vs '%s'" % (r1.id, r2.id)
            elif format=="clustal":
                assert r1.id.replace(" ","_")[:30] == r2.id, \
                       "'%s' vs '%s'" % (r1.id, r2.id)
            elif format=="stockholm":
                assert r1.id.replace(" ","_") == r2.id, \
                       "'%s' vs '%s'" % (r1.id, r2.id)
            elif format=="fasta":
                assert r1.id.split()[0] == r2.id
            else:
                assert r1.id == r2.id, \
                       "'%s' vs '%s'" % (r1.id, r2.id)

        if len(records)>1:
            #Try writing just one record (passing a SeqRecord, not a list)
            if format in SeqIO._BinaryFormats:
                handle = BytesIO()
            else:
                handle = StringIO()
            SeqIO.write(records[0], handle, format)
            assert handle.getvalue() == records[0].format(format)


#Check parsers can cope with an empty file
for t_format in SeqIO._FormatToIterator:
    if t_format in SeqIO._BinaryFormats or t_format=="uniprot-xml":
        #Not allowed empty SFF files.
        continue
    handle = StringIO()
    records = list(SeqIO.parse(handle, t_format))
    assert len(records) == 0

for (t_format, t_alignment, t_filename, t_count) in test_files:
    if t_format in SeqIO._BinaryFormats:
        mode = "rb"
    else:
        mode = "r"

    print "Testing reading %s format file %s" % (t_format, t_filename)
    assert os.path.isfile(t_filename), t_filename

    #Try as an iterator using handle
    h = open(t_filename,mode)
    records  = list(SeqIO.parse(handle=h, format=t_format))
    h.close()
    assert len(records)  == t_count, \
         "Found %i records but expected %i" % (len(records), t_count)

    #Try using the iterator with a for loop, and a filename not handle
    records2 = []
    for record in SeqIO.parse(t_filename, format=t_format):
        records2.append(record)
    assert len(records2) == t_count

    #Try using the iterator with the next() method
    records3 = []
    h = open(t_filename,mode)
    seq_iterator = SeqIO.parse(handle=h, format=t_format)
    while True:
        try:
            record = seq_iterator.next()
        except StopIteration:
            break
        assert record is not None, "Should raise StopIteration not return None"
        records3.append(record)
    h.close()

    #Try a mixture of next() and list (a torture test!)
    h = open(t_filename,mode)
    seq_iterator = SeqIO.parse(handle=h, format=t_format)
    try:
        record = seq_iterator.next()
    except StopIteration:
        record = None
    if record is not None:
        records4 = [record]
        records4.extend(list(seq_iterator))
    else:
        records4 = []
    assert len(records4) == t_count
    h.close()

    #Try a mixture of next() and for loop (a torture test!)
    #with a forward-only-handle
    if t_format == "abi":
        #Temp hack
        h = open(t_filename, mode)
    else:
        h = ForwardOnlyHandle(open(t_filename, mode))
    seq_iterator = SeqIO.parse(h, format=t_format)
    try:
        record = seq_iterator.next()
    except StopIteration:
        record = None
    if record is not None:
        records5 = [record]
        for record in seq_iterator:
            records5.append(record)
    else:
        records5 = []
    assert len(records5) == t_count
    h.close()

    for i in range(t_count):
        record = records[i]

        #Check returned expected object type
        assert isinstance(record, SeqRecord)
        if t_format in possible_unknown_seq_formats:
            assert isinstance(record.seq, Seq) or \
                   isinstance(record.seq, UnknownSeq)
        else:
            assert isinstance(record.seq, Seq)
        assert isinstance(record.id, basestring)
        assert isinstance(record.name, basestring)
        assert isinstance(record.description, basestring)
        assert record.id != ""

        if "accessions" in record.annotations:
            accs = record.annotations["accessions"]
            #Check for blanks, or entries with leading/trailing spaces
            for acc in accs:
                assert acc and acc == acc.strip(), \
                    "Bad accession in annotations: %s" % repr(acc)
            assert len(set(accs)) == len(accs), \
                   "Repeated accession in annotations: %s" % repr(accs)
        for ref in record.dbxrefs:
            assert ref and ref == ref.strip(), \
                "Bad cross reference in dbxrefs: %s" % repr(ref)
        assert len(record.dbxrefs) == len(record.dbxrefs), \
               "Repeated cross reference in dbxrefs: %s" % repr(record.dbxrefs)


        #Check the lists obtained by the different methods agree
        assert compare_record(record, records2[i])
        assert compare_record(record, records3[i])
        assert compare_record(record, records4[i])
        assert compare_record(record, records5[i])

        if i < 3:
            print record_summary(record)
    # Only printed the only first three records: 0,1,2
    if t_count > 4:
        print " ..."
    if t_count > 3:
        print record_summary(records[-1])

    # Check Bio.SeqIO.read(...)
    if t_count == 1:
        record = SeqIO.read(handle=open(t_filename,mode), format=t_format)
        assert isinstance(record, SeqRecord)
    else:
        try:
            record = SeqIO.read(open(t_filename), t_format)
            assert False, "Bio.SeqIO.read(...) should have failed"
        except ValueError:
            #Expected to fail
            pass

    # Check alphabets
    for record in records:
        base_alpha = Alphabet._get_base_alphabet(record.seq.alphabet)
        assert isinstance(base_alpha, Alphabet.SingleLetterAlphabet)
        if t_format in no_alpha_formats:
            assert base_alpha == Alphabet.single_letter_alphabet # Too harsh?
    if base_alpha is None:
        good = []
        bad =[]
        given_alpha=None
    elif isinstance(base_alpha, Alphabet.ProteinAlphabet):
        good = protein_alphas
        bad = dna_alphas + rna_alphas + nucleotide_alphas
    elif isinstance(base_alpha, Alphabet.RNAAlphabet):
        good = nucleotide_alphas + rna_alphas
        bad = protein_alphas + dna_alphas
    elif isinstance(base_alpha, Alphabet.DNAAlphabet):
        good = nucleotide_alphas + dna_alphas
        bad = protein_alphas + rna_alphas
    elif isinstance(base_alpha, Alphabet.NucleotideAlphabet):
        good = nucleotide_alphas
        bad = protein_alphas
    else:
        assert t_format in no_alpha_formats, "Got %s from %s file" \
               % (repr(base_alpha), t_format)
        good = protein_alphas + dna_alphas + rna_alphas + nucleotide_alphas
        bad = []
    for given_alpha in good:
        #These should all work...
        given_base = Alphabet._get_base_alphabet(given_alpha)
        for record in SeqIO.parse(open(t_filename,mode),t_format,given_alpha):
            base_alpha = Alphabet._get_base_alphabet(record.seq.alphabet)
            assert isinstance(base_alpha, given_base.__class__)
            assert base_alpha == given_base
        if t_count == 1:
            record = SeqIO.read(open(t_filename,mode),t_format,given_alpha)
            assert isinstance(base_alpha, given_base.__class__)
            assert base_alpha == given_base
    for given_alpha in bad:
        #These should all fail...
        try:
            print SeqIO.parse(open(t_filename,mode),t_format,given_alpha).next()
            assert False, "Forcing wrong alphabet, %s, should fail (%s)" \
                   % (repr(given_alpha), t_filename)
        except ValueError:
            pass
    del good, bad, given_alpha, base_alpha

    if t_alignment:
        print "Testing reading %s format file %s as an alignment" \
              % (t_format, t_filename)

        alignment = MultipleSeqAlignment(SeqIO.parse( \
                    handle=open(t_filename,mode), format=t_format))
        assert len(alignment) == t_count

        alignment_len = alignment.get_alignment_length()

        #Check the record order agrees, and double check the
        #sequence lengths all agree too.
        for i in range(t_count):
            assert compare_record(records[i], alignment[i])
            assert len(records[i].seq) == alignment_len

        print alignment_summary(alignment)

    #Some alignment file formats have magic characters which mean
    #use the letter in this position in the first sequence.
    #They should all have been converted by the parser, but if
    #not reversing the record order might expose an error.  Maybe.
    records.reverse()
    check_simple_write_read(records)

print "Finished tested reading files"

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